Journal: BMC Gastroenterology

Article Title: FCRL3 as a potential link between Benzo[a]pyrene exposure and primary biliary cholangitis: insights from comparative toxicogenomics and multi-omics analysis

doi: 10.1186/s12876-026-04614-x

Figure Lengend Snippet: Study design. BaP, benzo[a]pyrene; PBC, primary biliary cholangitis; SMR, summary data-based Mendelian randomization; QTL, quantitative trait locus; PPI, protein-protein interaction; KEGG, kyoto encyclopedia of genes and genomes; GO, gene ontology enrichment analysis; CETSA, cellular thermal shift assay

Article Snippet: Raji cells were seeded in specific medium and treated with various concentrations of Benzo[a]pyrene (HY-107377, MedChemExpress, China) at 0, 5, 10, 20, 40, and 80μM for 24 h. Following the incubation, the cells were harvested by centrifugation at 1000 × g for 5 min and washed twice with ice-cold phosphate-buffered saline (PBS) for subsequent experiments.

Techniques: Thermal Shift Assay

Journal: BMC Gastroenterology

Article Title: FCRL3 as a potential link between Benzo[a]pyrene exposure and primary biliary cholangitis: insights from comparative toxicogenomics and multi-omics analysis

doi: 10.1186/s12876-026-04614-x

Figure Lengend Snippet: A Molecular structure of BaP. B Venn diagram showing overlapping genes between BaP targets and PBC. C Causal relationship between BaP target genes expression and PBC. Expression level results by Benjamini-Hochberg correction and the HEterogeneity in dependent instruments test. BaP, benzo[a]pyrene; PBC, primary biliary cholangitis; OR, odds ratio; CI, confidence interval; HEIDI, HEterogeneity in dependent instruments

Article Snippet: Raji cells were seeded in specific medium and treated with various concentrations of Benzo[a]pyrene (HY-107377, MedChemExpress, China) at 0, 5, 10, 20, 40, and 80μM for 24 h. Following the incubation, the cells were harvested by centrifugation at 1000 × g for 5 min and washed twice with ice-cold phosphate-buffered saline (PBS) for subsequent experiments.

Techniques: Expressing

Journal: BMC Gastroenterology

Article Title: FCRL3 as a potential link between Benzo[a]pyrene exposure and primary biliary cholangitis: insights from comparative toxicogenomics and multi-omics analysis

doi: 10.1186/s12876-026-04614-x

Figure Lengend Snippet: A Causal relationship between genes methylation and PBC. B Causal relationship between proteins and PBC. Methylation and protein level results by Benjamini-Hochberg correction and the HEIDI test. C Docking between BaP and the predicted structure of the FCRL3 protein. PBC, primary biliary cholangitis; BaP, Benzo[a]pyrene; OR, odds ratio; CI, confidence interval; HEIDI, HEterogeneity in dependent instruments

Article Snippet: Raji cells were seeded in specific medium and treated with various concentrations of Benzo[a]pyrene (HY-107377, MedChemExpress, China) at 0, 5, 10, 20, 40, and 80μM for 24 h. Following the incubation, the cells were harvested by centrifugation at 1000 × g for 5 min and washed twice with ice-cold phosphate-buffered saline (PBS) for subsequent experiments.

Techniques: Methylation

Journal: BMC Gastroenterology

Article Title: FCRL3 as a potential link between Benzo[a]pyrene exposure and primary biliary cholangitis: insights from comparative toxicogenomics and multi-omics analysis

doi: 10.1186/s12876-026-04614-x

Figure Lengend Snippet: A Analysis of single-cell transcriptome data from HC and PBC liver tissues. B Violin plots of FCRL3 expression in different cell clusters. C Relative FCRL3 mRNA levels in Raji cells stimulated with BaP for 24 h. D FCRL3 protein levels in Raji cells stimulated with BaP for 24 h. β-actin was used as a loading control. E CETSA analysis to verify BaP binding to FCRL3 in Raji cells. Data are shown as mean ± SD, n = 3. Statistical analysis was performed using one-way ANOVA followed by Tukey’s post hoc test for RT-qPCR data, and CETSA melting curves were analyzed by nonlinear regression with differences assessed using two-way ANOVA. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001. HC, healthy control; PBC, primary biliary cholangitis; BaP, benzo[a]pyrene

Article Snippet: Raji cells were seeded in specific medium and treated with various concentrations of Benzo[a]pyrene (HY-107377, MedChemExpress, China) at 0, 5, 10, 20, 40, and 80μM for 24 h. Following the incubation, the cells were harvested by centrifugation at 1000 × g for 5 min and washed twice with ice-cold phosphate-buffered saline (PBS) for subsequent experiments.

Techniques: Single Cell, Expressing, Control, Binding Assay, Quantitative RT-PCR